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Sla I (Xho I isoschizomer)
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Sla I

(Xho I isoschizomer)

 Technical Brochure Technical Brochure
SlaI is a restriction enzyme purified from Stremptomyces lavendulae.

 

Unit substrate: Lambda DNA (HindIII digest).

 

Unit calculation assay conditions: 150 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25oC), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37oC.

 

Absence of contaminants: 400 units of Sla I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA (Hind III digest) at 37oC. After 100-fold overdigestion with SlaI, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.

 

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20oC.

 

Heat inactivation: 65oC for 20 minutes.

 

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Impaired

 

Percent Activity in MINOTECH Buffers
 L M  H  SH  A  K 
 25-50 75  75-100  100  10-25  100 
 
 
General reaction mixture:
10U SlaI 1μl
10x SH or K buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  
*In the case of SH buffer we recommend the addition of BSA to a final concentration of 100 μg/ml. 

 

Reagents supplied: 10x SH and 10x K buffer