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Sal I
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Sal I Technical Brochure Technical Brochure
 SalI is a restriction enzyme purified from Streptomyces albus G.

 

Unit substrate: Lambda DNA (HindIII digest).

 

Unit calculation assay conditions: 150 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C.

 

Absence of contaminants: 400 units of Sal I incubated for 16 hours at 37ºC with 1 μg of λ DNA (HindIII digest) resulted in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. After 50-fold overdigestion with Sal I, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

 

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 300 μg/ml bovine serum albumin and 50% glycerol. Store at -20oC.

 

Heat inactivation: 65oC for 20 minutes.

 

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Blocked

 

Star activity: Large excess of the enzyme results in the appearance of star activity.

 

Percent Activity in MINOTECH Buffers
 L M  H  SH  A  K 
 <10 25-50  50  100  <10  50 
 
 
General reaction mixture:
10U Sal I 1μl
10x SH buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  
*We recommend the addition of BSA to a final concentration of 100 μg/ml. 

 

Reagents supplied: 10x SH buffer