pUC18 DNA
(concentration 0.5μg/μl)

Description: pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC18 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC18 is 1.75x10⁶ daltons.

Preparation: pUC18 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.

Quality control: Gel analysis for purity. BsiSI and AluI fragmentation patterns.

Storage buffer: 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA. Store at -20^oC. 

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