BseCI is a restriction enzyme purified from Bacillus stearothermophilus.

Unit substrate: Lambda DNA.

Unit calculation assay conditions: 100 mM NaCl, 50 mM Tris-HCl  (pH 7.9 @ 25°C), 10  mM  MgCl₂, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 55^oC.

Absence of contaminants: 150 units of BseCI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 55^oC. After 100-fold overdigestion with BseCI greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100, 200 μg/ml BSA and 50% glycerol. Store at -20^oC.

Heat inactivation: No.

Methylation Sensitivity:

dam methylation: Blocked by overlapping

dcm methylation: Not sensitive

CpG methylation: Blocked

Reference: Rina, M., Dialektakis, D., Clark, D., Pagomenou, M. and Bouriotis, V. (1992). Nucleic Acids Res. 20

Reagents supplied: 10x H and 10x K buffer