* Size:

BamH I
Technical BrochureTechnical Brochure

BamHI is a restriction enzyme purified from Bacillus amyloliquefaciens H.


Unit substrate: Lambda DNA.


Unit calculation assay conditions: 100 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37oC.


Absence of contaminants: 100 units of BamHI incubated for 16 hours at 37oC with 1 μg of λ DNA resulted in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. After 50-fold overdigestion with BamHI, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.


Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20oC.


Heat inactivation: 80oC for 20 minutes.



Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive


Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration>5% or pH>8.0 may result in star activity.

Percent Activity in MINOTECH Buffers
 L SH 
 75 75-100 100 50-75 75 100


General reaction mixture:
10U BamH I
10x UBamHI or K buffer * 2μl 
DNA substrate 


Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  

*In the case of UBamHI buffer we recommend the addition
of BSA to a final concentration of 100 μg/ml.

Reagents supplied: 10x UBamHI and 10x K buffer